Deputies charged Lonnie Locklear, Trent Locklear, and Debra Locklear with 49 misdemeanor counts of Animal Cruelty.
Investigators say the Locklears are responsible for the care of the animals.
The arrests follow the seizure of 49 animals from a farm on Lonnie Farm Road in the Pembroke community of Robeson County nearly two weeks ago.
Deputies seized 26 horses, 14 cows, one pig, and one bull from the home on April 12.
Someone doing utility work near the farm saw a horse down and called authorities.
When officers got to the home, the horse was dead, deputies said.
Officers say two others had to be put down because they were in bad shape.
They say several carcasses of animals were also found on the property.
Deputies say pictures collected from the scene show the animals were deprived of necessary sustenance.
They say the animals have been placed in foster care pending the outcome of this investigation.
The Locklears have been released from the Robeson County Detention Center on a promise to appear in Lumberton District Court on May 29.